Abstract Title
Prediction of Serotypes by Molecular Phylogenetic Analysis of Enteric Infectious Viruses
Presenter
Kana Kidera, Laboratory of Viral Infection Control, Ōmura Satoshi Memorial Institute, Graduate School of Infection Control Sciences, Kitasato University
Co-Author(s)
Kana Kidera(1), Miyu Kameda(1), Yuya Fukuda(2), Hirokazu Kimura(3), Kei Haga(1), Reiko
Takai-Todaka(1), and Kazuhiko Katayama(1)
(1) Laboratory of Viral Infection Control, Ōmura Satoshi Memorial Institute, Graduate School of
Infection Control Sciences, Kitasato University
(2) Department of Pediatrics, Sapporo Medical University
(3) Department of Health Sciences, Gunma Paz University Graduate School
Abstract Category
Molecular Epidemiology & Evolution
Abstract
[Purpose]
Human norovirus (HuNoV) and human sapovirus (HuSaV), both members of Caliciviridae, are genetically diverse and classified into multiple genogroups and genotypes. For vaccine development, understanding whether these genetic classifications correspond to serotypes is essential. This study aimed to compare the genetic characteristics of HuNoV and HuSaV to assess their potential correlation with serotype classification.
[Methods]
Genome sequences of prototype strains and various genotypes were obtained from public databases. Multiple sequence alignments were performed using MAFFT and Clustal Omega. Genetic distances and amino acid identity were calculated, and phylogenetic trees were constructed using RAxML and NJ methods. Clusters were defined based on frequency distributions derived from amino acid identity matrices.
[Results and Discussion]
HuNoV genotype clusters had 49–83% amino acid identity, while genogroup clusters ranged from 39–49%. For HuSaV, genotype clusters showed 65–85% identity, and genogroup clusters 39–56%. These findings suggest that HuSaV genotypes are more conserved than those of HuNoV, potentially indicating stable antigenicity within genogroups. This raises the possibility that each HuSaV genogroup may correspond to a single serotype. Future work will involve virus neutralization assays using antisera against different HuSaV genotypes and genogroups, along with a newly established HuSaV-susceptible Caco-2MC cell line, to evaluate the relationship between genetic and serotype classifications.
Human norovirus (HuNoV) and human sapovirus (HuSaV), both members of Caliciviridae, are genetically diverse and classified into multiple genogroups and genotypes. For vaccine development, understanding whether these genetic classifications correspond to serotypes is essential. This study aimed to compare the genetic characteristics of HuNoV and HuSaV to assess their potential correlation with serotype classification.
[Methods]
Genome sequences of prototype strains and various genotypes were obtained from public databases. Multiple sequence alignments were performed using MAFFT and Clustal Omega. Genetic distances and amino acid identity were calculated, and phylogenetic trees were constructed using RAxML and NJ methods. Clusters were defined based on frequency distributions derived from amino acid identity matrices.
[Results and Discussion]
HuNoV genotype clusters had 49–83% amino acid identity, while genogroup clusters ranged from 39–49%. For HuSaV, genotype clusters showed 65–85% identity, and genogroup clusters 39–56%. These findings suggest that HuSaV genotypes are more conserved than those of HuNoV, potentially indicating stable antigenicity within genogroups. This raises the possibility that each HuSaV genogroup may correspond to a single serotype. Future work will involve virus neutralization assays using antisera against different HuSaV genotypes and genogroups, along with a newly established HuSaV-susceptible Caco-2MC cell line, to evaluate the relationship between genetic and serotype classifications.